The reduced form of nicotinamide adenine dinucleotide (NADH)
is an important and highly fluorescent coenzyme. It has an absorption maximum
of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave
the following fluorescence intensities:
(a) Construct a spreadsheet and use it to draw a calibration
curve for NADH.
*(b) Find the least-squares slope and intercept for the plot
(c) Calculate the standard deviation of the slope and the
standard deviation about regression for the curve.
*(d) An unknown exhibits a relative fluorescence intensity
of 12.16. Use the spreadsheet to calculate the concentration of NADH.
*(e) Calculate the relative standard deviation for the
result in part (d).
*(f) Calculate the relative standard deviation for the
result in part (d) if a result of 7.95 was the mean of three measurements.